Laboratory evaluation of transgenic Populus davidiana×Populus bolleana expressing Cry1Ac + SCK, Cry1Ah3, and Cry9Aa3 genes against gypsy moth and fall webworm.
Identifieur interne : 001319 ( Main/Exploration ); précédent : 001318; suivant : 001320Laboratory evaluation of transgenic Populus davidiana×Populus bolleana expressing Cry1Ac + SCK, Cry1Ah3, and Cry9Aa3 genes against gypsy moth and fall webworm.
Auteurs : Liping Ding [République populaire de Chine] ; Yajuan Chen [République populaire de Chine] ; Xiaoli Wei [République populaire de Chine] ; Mi Ni [République populaire de Chine] ; Jiewei Zhang [République populaire de Chine] ; Hongzhi Wang [République populaire de Chine] ; Zhen Zhu [République populaire de Chine] ; Jianhua Wei [République populaire de Chine]Source :
- PloS one [ 1932-6203 ] ; 2017.
Descripteurs français
- KwdFr :
- Agrobacterium tumefaciens (génétique), Agrobacterium tumefaciens (métabolisme), Amélioration des plantes (MeSH), Animaux (MeSH), Croisements génétiques (MeSH), Endotoxines (génétique), Endotoxines (immunologie), Expression des gènes (MeSH), Feuilles de plante (génétique), Feuilles de plante (immunologie), Feuilles de plante (parasitologie), Hémolysines (génétique), Hémolysines (immunologie), Immunité des plantes (génétique), Isoformes de protéines (génétique), Isoformes de protéines (immunologie), Larve (croissance et développement), Lepidoptera (croissance et développement), Lutte biologique contre les nuisibles (méthodes), Papillons de nuit (croissance et développement), Populus (génétique), Populus (immunologie), Populus (parasitologie), Protéines bactériennes (génétique), Protéines bactériennes (immunologie), Transformation génétique (MeSH), Transgènes (MeSH), Végétaux génétiquement modifiés (MeSH).
- MESH :
- croissance et développement : Larve, Lepidoptera, Papillons de nuit.
- génétique : Agrobacterium tumefaciens, Endotoxines, Feuilles de plante, Hémolysines, Immunité des plantes, Isoformes de protéines, Populus, Protéines bactériennes.
- immunologie : Endotoxines, Feuilles de plante, Hémolysines, Isoformes de protéines, Populus, Protéines bactériennes.
- métabolisme : Agrobacterium tumefaciens.
- méthodes : Lutte biologique contre les nuisibles.
- parasitologie : Feuilles de plante, Populus.
- Amélioration des plantes, Animaux, Croisements génétiques, Expression des gènes, Transformation génétique, Transgènes, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- Agrobacterium tumefaciens (genetics), Agrobacterium tumefaciens (metabolism), Animals (MeSH), Bacterial Proteins (genetics), Bacterial Proteins (immunology), Crosses, Genetic (MeSH), Endotoxins (genetics), Endotoxins (immunology), Gene Expression (MeSH), Hemolysin Proteins (genetics), Hemolysin Proteins (immunology), Larva (growth & development), Lepidoptera (growth & development), Moths (growth & development), Pest Control, Biological (methods), Plant Breeding (MeSH), Plant Immunity (genetics), Plant Leaves (genetics), Plant Leaves (immunology), Plant Leaves (parasitology), Plants, Genetically Modified (MeSH), Populus (genetics), Populus (immunology), Populus (parasitology), Protein Isoforms (genetics), Protein Isoforms (immunology), Transformation, Genetic (MeSH), Transgenes (MeSH).
- MESH :
- chemical , genetics : Bacterial Proteins, Endotoxins, Hemolysin Proteins, Protein Isoforms.
- genetics : Agrobacterium tumefaciens, Plant Immunity, Plant Leaves, Populus.
- growth & development : Larva, Lepidoptera, Moths.
- chemical , immunology : Bacterial Proteins, Endotoxins, Hemolysin Proteins, Plant Leaves, Populus, Protein Isoforms.
- metabolism : Agrobacterium tumefaciens.
- methods : Pest Control, Biological.
- parasitology : Plant Leaves, Populus.
- Animals, Crosses, Genetic, Gene Expression, Plant Breeding, Plants, Genetically Modified, Transformation, Genetic, Transgenes.
Abstract
Transgenic poplar lines 'Shanxin' (Populus davidiana×Populus bolleana) were generated via Agrobacterium-mediated transformation. The transgenic lines carried the expression cassettes of Cry1Ac + SCK, Cry1Ah3, and Cry9Aa3, respectively. The expression levels of the exogenous insect resistance genes in the transgenic lines were determined by Q-PCR and Western blot. Leaves of the transgenic lines were used for insect feeding bioassays on first instar larvae of the gypsy moth (Lymantria dispar) and fall webworm (Hyphantria cunea). At 5 d of feeding, the mean mortalities of larvae feeding on Cry1Ac + SCK and Cry1Ah3 transgenic poplars leaves were 97% and 91%, while mortality on Cry9Aa3 transgenic lines was about 49%. All gypsy moth and fall webworm larvae were killed in 7-9 days after feeding on leaves from Cry1Ac + SCK or Cry1Ah3 transgenic poplars, while all the fall webworm larvae were killed in 11 days and about 80% of gypsy moth larvae were dead in 14 days after feeding on those from Cry9Aa3 transgenic lines. It was concluded that the transgenic lines of Cry1Ac + SCK and Cry1Ah3 were highly toxic to larvae of both insect species while lines with Cry9Aa3 had lower toxicity,and H. cunea larvae are more sensitive to the insecticidal proteins compared to L. dispar. Transgenic poplar lines toxic to L. dispar and H. cunea could be used to provide Lepidoptera pest resistance to selected strains of poplar trees.
DOI: 10.1371/journal.pone.0178754
PubMed: 28582405
PubMed Central: PMC5459438
Affiliations:
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R. China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>Beijing Key Laboratory of Agriculture Gene Resource and Biotechnology, Beijing Academy of Agriculture and Forestry Sciences, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Chen, Yajuan" sort="Chen, Yajuan" uniqKey="Chen Y" first="Yajuan" last="Chen">Yajuan Chen</name><affiliation wicri:level="3"><nlm:affiliation>Beijing Key Laboratory of Agriculture Gene Resource and Biotechnology, Beijing Academy of Agriculture and Forestry Sciences, Beijing, P. R. China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>Beijing Key Laboratory of Agriculture Gene Resource and Biotechnology, Beijing Academy of Agriculture and Forestry Sciences, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Wei, Xiaoli" sort="Wei, Xiaoli" uniqKey="Wei X" first="Xiaoli" last="Wei">Xiaoli Wei</name><affiliation wicri:level="3"><nlm:affiliation>State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, P. R. China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Ni, Mi" sort="Ni, Mi" uniqKey="Ni M" first="Mi" last="Ni">Mi Ni</name><affiliation wicri:level="3"><nlm:affiliation>State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, P. R. China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zhang, Jiewei" sort="Zhang, Jiewei" uniqKey="Zhang J" first="Jiewei" last="Zhang">Jiewei Zhang</name><affiliation wicri:level="3"><nlm:affiliation>Beijing Key Laboratory of Agriculture Gene Resource and Biotechnology, Beijing Academy of Agriculture and Forestry Sciences, Beijing, P. R. China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>Beijing Key Laboratory of Agriculture Gene Resource and Biotechnology, Beijing Academy of Agriculture and Forestry Sciences, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Wang, Hongzhi" sort="Wang, Hongzhi" uniqKey="Wang H" first="Hongzhi" last="Wang">Hongzhi Wang</name><affiliation wicri:level="3"><nlm:affiliation>Beijing Key Laboratory of Agriculture Gene Resource and Biotechnology, Beijing Academy of Agriculture and Forestry Sciences, Beijing, P. R. China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>Beijing Key Laboratory of Agriculture Gene Resource and Biotechnology, Beijing Academy of Agriculture and Forestry Sciences, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zhu, Zhen" sort="Zhu, Zhen" uniqKey="Zhu Z" first="Zhen" last="Zhu">Zhen Zhu</name><affiliation wicri:level="3"><nlm:affiliation>State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, P. R. China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Wei, Jianhua" sort="Wei, Jianhua" uniqKey="Wei J" first="Jianhua" last="Wei">Jianhua Wei</name><affiliation wicri:level="3"><nlm:affiliation>Beijing Key Laboratory of Agriculture Gene Resource and Biotechnology, Beijing Academy of Agriculture and Forestry Sciences, Beijing, P. R. China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>Beijing Key Laboratory of Agriculture Gene Resource and Biotechnology, Beijing Academy of Agriculture and Forestry Sciences, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author></analytic><series><title level="j">PloS one</title><idno type="eISSN">1932-6203</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Agrobacterium tumefaciens (genetics)</term><term>Agrobacterium tumefaciens (metabolism)</term><term>Animals (MeSH)</term><term>Bacterial Proteins (genetics)</term><term>Bacterial Proteins (immunology)</term><term>Crosses, Genetic (MeSH)</term><term>Endotoxins (genetics)</term><term>Endotoxins (immunology)</term><term>Gene Expression (MeSH)</term><term>Hemolysin Proteins (genetics)</term><term>Hemolysin Proteins (immunology)</term><term>Larva (growth & development)</term><term>Lepidoptera (growth & development)</term><term>Moths (growth & development)</term><term>Pest Control, Biological (methods)</term><term>Plant Breeding (MeSH)</term><term>Plant Immunity (genetics)</term><term>Plant Leaves (genetics)</term><term>Plant Leaves (immunology)</term><term>Plant Leaves (parasitology)</term><term>Plants, Genetically Modified (MeSH)</term><term>Populus (genetics)</term><term>Populus (immunology)</term><term>Populus (parasitology)</term><term>Protein Isoforms (genetics)</term><term>Protein Isoforms (immunology)</term><term>Transformation, Genetic (MeSH)</term><term>Transgenes (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Agrobacterium tumefaciens (génétique)</term><term>Agrobacterium tumefaciens (métabolisme)</term><term>Amélioration des plantes (MeSH)</term><term>Animaux (MeSH)</term><term>Croisements génétiques (MeSH)</term><term>Endotoxines (génétique)</term><term>Endotoxines (immunologie)</term><term>Expression des gènes (MeSH)</term><term>Feuilles de plante (génétique)</term><term>Feuilles de plante (immunologie)</term><term>Feuilles de plante (parasitologie)</term><term>Hémolysines (génétique)</term><term>Hémolysines (immunologie)</term><term>Immunité des plantes (génétique)</term><term>Isoformes de protéines (génétique)</term><term>Isoformes de protéines (immunologie)</term><term>Larve (croissance et développement)</term><term>Lepidoptera (croissance et développement)</term><term>Lutte biologique contre les nuisibles (méthodes)</term><term>Papillons de nuit (croissance et développement)</term><term>Populus (génétique)</term><term>Populus (immunologie)</term><term>Populus (parasitologie)</term><term>Protéines bactériennes (génétique)</term><term>Protéines bactériennes (immunologie)</term><term>Transformation génétique (MeSH)</term><term>Transgènes (MeSH)</term><term>Végétaux génétiquement modifiés (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Bacterial Proteins</term><term>Endotoxins</term><term>Hemolysin Proteins</term><term>Protein Isoforms</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Larve</term><term>Lepidoptera</term><term>Papillons de nuit</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Agrobacterium tumefaciens</term><term>Plant Immunity</term><term>Plant Leaves</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Larva</term><term>Lepidoptera</term><term>Moths</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Agrobacterium tumefaciens</term><term>Endotoxines</term><term>Feuilles de plante</term><term>Hémolysines</term><term>Immunité des plantes</term><term>Isoformes de protéines</term><term>Populus</term><term>Protéines bactériennes</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Endotoxines</term><term>Feuilles de plante</term><term>Hémolysines</term><term>Isoformes de protéines</term><term>Populus</term><term>Protéines bactériennes</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Bacterial Proteins</term><term>Endotoxins</term><term>Hemolysin Proteins</term><term>Plant Leaves</term><term>Populus</term><term>Protein Isoforms</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Agrobacterium tumefaciens</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Pest Control, Biological</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Agrobacterium tumefaciens</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Lutte biologique contre les nuisibles</term></keywords><keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Feuilles de plante</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Plant Leaves</term><term>Populus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Crosses, Genetic</term><term>Gene Expression</term><term>Plant Breeding</term><term>Plants, Genetically Modified</term><term>Transformation, Genetic</term><term>Transgenes</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Amélioration des plantes</term><term>Animaux</term><term>Croisements génétiques</term><term>Expression des gènes</term><term>Transformation génétique</term><term>Transgènes</term><term>Végétaux génétiquement modifiés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Transgenic poplar lines 'Shanxin' (Populus davidiana×Populus bolleana) were generated via Agrobacterium-mediated transformation. The transgenic lines carried the expression cassettes of Cry1Ac + SCK, Cry1Ah3, and Cry9Aa3, respectively. The expression levels of the exogenous insect resistance genes in the transgenic lines were determined by Q-PCR and Western blot. Leaves of the transgenic lines were used for insect feeding bioassays on first instar larvae of the gypsy moth (Lymantria dispar) and fall webworm (Hyphantria cunea). At 5 d of feeding, the mean mortalities of larvae feeding on Cry1Ac + SCK and Cry1Ah3 transgenic poplars leaves were 97% and 91%, while mortality on Cry9Aa3 transgenic lines was about 49%. All gypsy moth and fall webworm larvae were killed in 7-9 days after feeding on leaves from Cry1Ac + SCK or Cry1Ah3 transgenic poplars, while all the fall webworm larvae were killed in 11 days and about 80% of gypsy moth larvae were dead in 14 days after feeding on those from Cry9Aa3 transgenic lines. It was concluded that the transgenic lines of Cry1Ac + SCK and Cry1Ah3 were highly toxic to larvae of both insect species while lines with Cry9Aa3 had lower toxicity,and H. cunea larvae are more sensitive to the insecticidal proteins compared to L. dispar. Transgenic poplar lines toxic to L. dispar and H. cunea could be used to provide Lepidoptera pest resistance to selected strains of poplar trees.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">28582405</PMID><DateCompleted><Year>2017</Year><Month>09</Month><Day>18</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Electronic">1932-6203</ISSN><JournalIssue CitedMedium="Internet"><Volume>12</Volume><Issue>6</Issue><PubDate><Year>2017</Year></PubDate></JournalIssue><Title>PloS one</Title><ISOAbbreviation>PLoS One</ISOAbbreviation></Journal><ArticleTitle>Laboratory evaluation of transgenic Populus davidiana×Populus bolleana expressing Cry1Ac + SCK, Cry1Ah3, and Cry9Aa3 genes against gypsy moth and fall webworm.</ArticleTitle><Pagination><MedlinePgn>e0178754</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1371/journal.pone.0178754</ELocationID><Abstract><AbstractText>Transgenic poplar lines 'Shanxin' (Populus davidiana×Populus bolleana) were generated via Agrobacterium-mediated transformation. The transgenic lines carried the expression cassettes of Cry1Ac + SCK, Cry1Ah3, and Cry9Aa3, respectively. The expression levels of the exogenous insect resistance genes in the transgenic lines were determined by Q-PCR and Western blot. Leaves of the transgenic lines were used for insect feeding bioassays on first instar larvae of the gypsy moth (Lymantria dispar) and fall webworm (Hyphantria cunea). At 5 d of feeding, the mean mortalities of larvae feeding on Cry1Ac + SCK and Cry1Ah3 transgenic poplars leaves were 97% and 91%, while mortality on Cry9Aa3 transgenic lines was about 49%. All gypsy moth and fall webworm larvae were killed in 7-9 days after feeding on leaves from Cry1Ac + SCK or Cry1Ah3 transgenic poplars, while all the fall webworm larvae were killed in 11 days and about 80% of gypsy moth larvae were dead in 14 days after feeding on those from Cry9Aa3 transgenic lines. It was concluded that the transgenic lines of Cry1Ac + SCK and Cry1Ah3 were highly toxic to larvae of both insect species while lines with Cry9Aa3 had lower toxicity,and H. cunea larvae are more sensitive to the insecticidal proteins compared to L. dispar. Transgenic poplar lines toxic to L. dispar and H. cunea could be used to provide Lepidoptera pest resistance to selected strains of poplar trees.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ding</LastName><ForeName>Liping</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Beijing Key Laboratory of Agriculture Gene Resource and Biotechnology, Beijing Academy of Agriculture and Forestry Sciences, Beijing, P. R. China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Yajuan</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Beijing Key Laboratory of Agriculture Gene Resource and Biotechnology, Beijing Academy of Agriculture and Forestry Sciences, Beijing, P. R. China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wei</LastName><ForeName>Xiaoli</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, P. R. China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ni</LastName><ForeName>Mi</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, P. R. China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Jiewei</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Beijing Key Laboratory of Agriculture Gene Resource and Biotechnology, Beijing Academy of Agriculture and Forestry Sciences, Beijing, P. R. China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Hongzhi</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Beijing Key Laboratory of Agriculture Gene Resource and Biotechnology, Beijing Academy of Agriculture and Forestry Sciences, Beijing, P. R. China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhu</LastName><ForeName>Zhen</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, P. R. China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wei</LastName><ForeName>Jianhua</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Beijing Key Laboratory of Agriculture Gene Resource and Biotechnology, Beijing Academy of Agriculture and Forestry Sciences, Beijing, P. R. China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>06</Month><Day>05</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>PLoS One</MedlineTA><NlmUniqueID>101285081</NlmUniqueID><ISSNLinking>1932-6203</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D001426">Bacterial Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004731">Endotoxins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D006460">Hemolysin Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D020033">Protein Isoforms</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C030956">insecticidal crystal protein, Bacillus Thuringiensis</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D016960" MajorTopicYN="N">Agrobacterium tumefaciens</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001426" MajorTopicYN="N">Bacterial Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003433" MajorTopicYN="N">Crosses, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004731" MajorTopicYN="N">Endotoxins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015870" MajorTopicYN="N">Gene Expression</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006460" MajorTopicYN="N">Hemolysin Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007814" MajorTopicYN="N">Larva</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007915" MajorTopicYN="N">Lepidoptera</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009036" MajorTopicYN="N">Moths</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010572" MajorTopicYN="N">Pest Control, Biological</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000069600" MajorTopicYN="N">Plant Breeding</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D057865" MajorTopicYN="N">Plant Immunity</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000469" MajorTopicYN="N">parasitology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="Y">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000469" MajorTopicYN="N">parasitology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020033" MajorTopicYN="N">Protein Isoforms</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014170" MajorTopicYN="N">Transformation, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019076" MajorTopicYN="Y">Transgenes</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year><Month>09</Month><Day>12</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2017</Year><Month>05</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>6</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>6</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2017</Year><Month>9</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">28582405</ArticleId><ArticleId IdType="doi">10.1371/journal.pone.0178754</ArticleId><ArticleId IdType="pii">PONE-D-16-35196</ArticleId><ArticleId IdType="pmc">PMC5459438</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Curr Microbiol. 2011 Nov;63(5):420-5</Citation><ArticleIdList><ArticleId IdType="pubmed">21858696</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Rep. 1991 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Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Ding, Liping" sort="Ding, Liping" uniqKey="Ding L" first="Liping" last="Ding">Liping Ding</name></noRegion><name sortKey="Chen, Yajuan" sort="Chen, Yajuan" uniqKey="Chen Y" first="Yajuan" last="Chen">Yajuan Chen</name><name sortKey="Ni, Mi" sort="Ni, Mi" uniqKey="Ni M" first="Mi" last="Ni">Mi Ni</name><name sortKey="Wang, Hongzhi" sort="Wang, Hongzhi" uniqKey="Wang H" first="Hongzhi" last="Wang">Hongzhi Wang</name><name sortKey="Wei, Jianhua" sort="Wei, Jianhua" uniqKey="Wei J" first="Jianhua" last="Wei">Jianhua Wei</name><name sortKey="Wei, Xiaoli" sort="Wei, Xiaoli" uniqKey="Wei X" first="Xiaoli" last="Wei">Xiaoli Wei</name><name sortKey="Zhang, Jiewei" sort="Zhang, Jiewei" uniqKey="Zhang J" first="Jiewei" last="Zhang">Jiewei Zhang</name><name sortKey="Zhu, Zhen" sort="Zhu, Zhen" uniqKey="Zhu Z" first="Zhen" last="Zhu">Zhen 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